Supervisor Project : Role of protein Ubiquitylation in the Proteasome-Autophagy Crosstalk


Partner Lab


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Manuel S. Rodriguez obtained his first degrees from the National Autonomous University of Mexico (UNAM). After his Ph.D. in Microbiology at Paris 7 University/Pasteur Institute, most of his carrier has continued in Europe in distinct research centres including the Centre for Biomolecular Sciences of the University of St. Andrews (UK), Jacques Monod Institute-CNRS (France), CIC bioGUNE and Inbiomed (Spain).
He is currently based at the ITAV-IPBS (France).He is founder member of the European Network INPROTEOLYS and co-coordinator of the ITN–Marie Curie networks UPStream and UbiCODE, both dedicated to study of the ubiquitin-proteasome system.

Summary of the Project

Multiple aspects regarding the biological relevance of distinct Ub chains and their role in the connection of between the Proteasome and Autophagy pathways remain unexplored. To address these questions our group has developed molecular traps (Tandem Ub Binding Entities or TUBEs) to capture and identify proteins modified by members of the Ub family. Here we propose the use of new molecular traps based in UBD from proteins implicated in autophagy (e.g. p62): i) Ubiquitylated proteins will be identified using a TUBEs-MS approach; ii) To study their specificity, captured proteins will be compared to those proteins captured with distinct Ub traps; iii) Ubiquitylated proteins-UDB interactions will also be studied using small inhibitor molecules; iv) Role of identified factors in UPS-Autophagy crosstalk under proteotoxic stress conditions will be analysed.

Funding agencies