Ivan DIKIC
Supervisor Project : Linear chain ubiquitinome and its pharmacological targeting in inflammation and bacterial infection
Partner Lab
a strong profile in life sciences, smoothly integrating the full range of natural science
disciplines with the academic medical centre. GUF has a reputation for pursuing
interdisciplinary programs and has been very successful in securing funding for three
Excellence Clusters, two of them in the fields of natural/life sciences and translational
biomedicine. The Institute of Biochemistry II (IBC2) is a basic research institute situated
on the medical campus and sustaining an outstation on the natural science campus. This
setting has fuelled numerous interdisciplinary projects integrating structural biology,
functional studies and translational approaches.
About
Director of the Institute of Biochemistry II at Goethe University Frankfurt. He is also member of several academic societies, including the number of organizations including the European Molecular Biology Organization (since 2004), the World Academy of Art and Science (since 2008), German National Academy of Sciences Leopoldina (since 2010), the Croatian Academy of Medical Science, and the European Academy of Sciences.
Summary of the Project
By applying our recently developed MS method we identified global ubiquitinome of pathogenic Salmonella. We further demonstrated that linear ubiquitylation is required for activation of NF-kB at the surface of bacteria. Therefore, the first objective of this proposal is to investigate now the global linear Ub changes during inflammation responses and bacterial infection using cell culture models and primary cells. The second objective is to perform detailed functional characterization of newly identified pathways mediated by linear ubiquitylation. The third objective is to develop activity-based probes and a robust high-throughput assay platform for pharmacological inhibition of linear Ub ligases.