Supervisor Project : Expanding chemical technologies towards SUMO and NEDD8-based reagents. This project relates to all WPs, allowing the generation of a broadly applicable scale of reagents.
The Ub/UbL code is translated into biochemical action through interaction with specific binding domains. These domains specifically bind to Ub or UbLs that can be present in larger chains. In order to interfere with chain signalling, modulation of these interactions is an interesting but so far unexplored option. It is important to have good methods available that help determine where and when such domains interact. Currently we can efficiently generate a board scale of Ub-based research reagents based on chemical synthesis. This project aims to translate synthetic methods that allow the generation of Ub-based reagents, probes and chains towards chemical methods to do the same for UbL proteins, starting with SUMO-1, -2 and -3 as well as NEDD8. The resulting UbL reagents will then be used in collaboration with other members of the network to study Ub, SUMO and NEDD8 biology.