Objective: To train ESRs on protein biochemistry and on how to gain molecular insights into the enzymes and proteins of the Ub system.
Abstract: Ub interactions are at the core of the Ub system, and their understanding at the molecular level has propelled the field forward. In this course, ESRs will learn about the latest protein biochemistry techniques and methods to make Ub and study Ub interactions with biophysical and structural methods. The course will enable ESRs to understand the structural data available, and teach them what is needed to obtain similar data for their project.
Day 1: Introduction by all teachers, introduction of ESR projects.
Day 2: Theoretical: protein biochemistry, requirements for successful biophysics and structural studies, options for studying Ub binding, introduction to NMR; Practical: measuring Ub interactions by NMR.
Day 3: Theoretical: quantitative methods to measure Ub-protein interactions, introduction to fluorescence, FP, ITC, SPR. Introduction enzyme kinetics; Practical: Biophysics: measuring KD by FP and ITC, measuring DUB kinetics by FP.
Day 4: Theoretical: X-ray crystallography overview and introduction; how good is a structure? What can I do with it?; Practical: Handling protein structures with Pymol.